After the certain amount of ml per substance was mixed, the absorbency readings for the effect of peroxides concentration were taken from the spectrophotometer. The results for the effect of peroxides concentration experiment were found. The results for this lab were that the rate increased, the concentration increased as well. The next experiment was testing the effect of temperature on peroxides activity. 8 test tubes were needed with different amounts of pH 5, H2O, Peroxides, and Caligula. The test tube was kept at temperatures of 4, 23, 37, and 70 degrees Celsius.
After he test tubes took time to settle at the specific temperatures, the absorbency value at 500 NM as well as the results for the effect of temperature on the rate of peroxides were found. The results for the different temperatures was that the temperature was too low or too high, the proteins denatured, but when they were between room temperature and body temperature they thrived. The final experiment was testing the effect of pH on peroxides activity. The same substances as the previous two were used, but this time it was with the pH’s of 3, 5, 7, and 9.
The absorbency values at 500 NM and the exults for the effect of pH on the rate of peroxides were recorded. The results were similar to the second lab because when the pH was too low or too high the proteins denatured and the two middle pHs were optimal pH levels. INTRODUCTION The purpose of this experiment was to determine the effect of concentration, temperature, and pH on enzyme activity (Aerial et al. , 2006). Before diving further into the topic, one must understand: What is an enzyme? Enzymes are macromolecular biological catalysts that are responsible for thousands of metabolic processes that sustain life.
An enzyme s a three-dimensional globular protein that acts as a biological catalyst (Cambial, et al, 2008). They are highly selective catalysts which are responsible for speeding up the rate of reactions. According to the properties of enzymes lab, “life as we know it would be impossible without enzymes cataloging these reactions because, in their absence, they occur too slowly/’ (Various authors 2001). They speed up reactions by lowering the activation energy that is needed for the reaction to proceed. They do this by binding to specific substrates and forming enzyme substrate complexes.
Because an enzymes shape is critical to its performance, environmental factors which could change this shape could have an effect on enzyme production (Campbell, et al. 2008). After this they undergo conformational changes that increase the chances of starting the reaction. Once this is complete, the product is released and the enzyme is able to catalyst the same reaction. Though there are many types of organisms found in living organisms, the one studied in this experiment is peroxides. Peroxides oxidized various compounds using peroxides found in nature, especially hydrogen peroxide, which are reduced, forming water. Aerobic organisms produce a class of enzymes called peroxides that catalyst this chemical reaction in order to quickly eliminate H2O from cells and tissues to prevent its damaging effects” (Various Authors 2001). Each enzymatic reaction has its own set of optimum conditions which produce the most efficient rate of enzymatic reaction or fastest rate of reaction. These conditions vary based on the enzymes or the location of the reaction in the body. The most important factors that can affect the optimum conditions are temperature of reaction, concentration in he reaction, or the pH of the reaction.